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The antibody is from purified rabbit serum by affinity purification via sequential chromatography on phospho-peptide and non-phospho-peptide affinity columns.
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Overexpression of the gene has long been noticed in several tumors, which leads to chromosome instability, and so may possibly contribute to tumorigenesis. This protein was discovered to affiliate with, and be involved in, the phosphorylation of NPAT protein (nuclear protein mapped to the ATM locus), which participates in mobile-cycle regulated histone gene expression and performs a important part in advertising mobile-cycle progression within the absence of pRB. Two alternatively spliced transcript variants of the gene, which encode distinct isoforms, are described. Two additional splice variants ended up noted but in-depth nucleotide sequence details just isn't however accessible.
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Blocking peptides are peptides that bind exclusively to the goal antibody and block antibody binding. These peptide ordinarily is made up of the epitope acknowledged with the antibody.
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This guide outlines even more possible optimization of cellular and immunoassay parameters to make sure the very best outcomes are...
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This tutorial outlines further more probable optimization of mobile and immunoassay parameters to ensure the best possible success are...
Antibodies bound to the blocking peptide no longer bind to the epitope on the focus on protein. By comparing the staining from the blocked antibody vs . the antibody by itself, you can see which staining is distinct.
Focus on Details Cyclin more info E belongs for the extremely conserved cyclin family members, whose members are characterised by a spectacular periodicity in protein abundance throughout the cell cycle. Cyclins perform as regulators of CDK kinases. Different cyclins show unique expression and degradation patterns which add to your temporal coordination of every mitotic event. Cyclin E sorts a fancy with and functions to be a regulatory subunit of CDK2, whose action is required for mobile cycle G1/S transition. This protein accumulates at the G1-S period boundary and it is degraded as cells development by way of S stage.
The measurement of protein phosphorylation is a useful tool for measuring the modulation of receptor activation by both equally antibodies...
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